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Summary: Researchers have identified the cellular and molecular mechanisms that regulate selective autophagy.

sorkE: University of Warwick

The study of autophagy – the process of recycling and repair within cells – has tremendous potential to help fight the aging process, bacterial and viral infections, and diseases including cancer, Alzheimer’s disease and Parkinson’s disease.

A team of researchers led by Professor Ioannis Nezes from the School of Life Sciences at the University of Warwick has identified the molecular and cellular mechanisms that regulate selective autophagy in Drosophila. Drosophila black belly.

While the function of these processes is increasingly being understood in mammals, it is one of the first studies done in insects.

The study opens new avenues in our understanding of the Golgi complex turnover regulation by selective autophagy. The Golgi complex is a stack of flattened sacs made up of membranes inside the cell. It prepares proteins and fat molecules for transport and use elsewhere inside and outside the cell.

Professor Nezes and his team used genetic editing, and electron microscopy, to identify a new type of selective autophagy, called Golgiphagy, which means how cells degrade a cell organelle called the Golgi complex by autophagy.

in paper, GMAP is an Atg8a-inteacting protein that regulates Golgi turnover in Drosophila. Published today in the magazine cell reportsPhD students Ashraf Rahman, Raksha Guhel and their colleagues describe how gene editing has been used to create fruit flies unable to process certain proteins via autophagy.

The study opens new avenues in our understanding of the Golgi complex turnover regulation by selective autophagy. The image is in the public domain

A comparison of transgenic flies with their wild-type counterparts showed:-

  • This LDS docking site Atg8a is important in carrying out selective autophagy
  • This selective autophagy regulates the size and morphology of the Golgi apparatus
  • GMAP (Golgi microtubule-associated protein) interacts with Atg8a and the LIR element at position 320-325 is important for this interaction
  • The LIR model of GMAP is the key word of Golgiphagy

Lead author of the research Professor Ioannis Nezes from the School of Life Sciences at the University of Warwick said:

“Understanding the molecular mechanisms of selective Golgi autophagy in cells will help open up new avenues of research that will help elucidate the cellular mechanisms underlying diseases.”

About this autophagy research news

author: Sheila Kiggins
source: University of Warwick
Contact: Sheila Keggins – University of Warwick
picture: The image is in the public domain

original search: open access.
“GMAP is an Atg8a interacting protein that regulates Golgi turnover in Drosophila” by Ioannis Nezis et al. cell reports

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Summary

GMAP is an Atg8a interacting protein that regulates Golgi turnover in Drosophila

Highlights

  • Atg8a-LDS mutants aggregate autophagy substrates and reduce lifespan
  • Quantitative proteomics determines GMAP accumulation in Atg8a-LDS mutants
  • GMAP interacts with Atg8a via the LIR نموذج motif
  • Atg8a-LDS and GMAP LIR mutants show an elongated Golgi morphology

Summary

Selective autophagy receptors and adapters contain short linear motifs called LIR (LC3 interacting region) motifs, which are required for interaction with Atg8 family proteins. The LIR motifs bind to the hydrophobic pockets of the LIR docking site (LDS) of the respective Atg8 family proteins. The physiological significance of LDS docking sites has not been elucidated in vivo.

Here, we show that Atg8a-LDS is a mutant fruit fly Flies accumulate on autophagic substrates and reduce life span.

Using quantitative proteomics to identify proteins that accumulate in Atg8a-LDS mutants, we determine Commonwealth of Independent StatesGolgi protein GMAP (Golgi microtubule-associated protein) as a protein containing the LIR motif that interacts with Atg8a. GMAP LIR mutant flies show an accumulation of Golgi markers and an elongated Golgi morphology.

Our data indicate that GMAP mediates Golgi turnover by selective autophagy to regulate its morphology and size via its LIR-mediated interaction with Atg8a.

2022-06-05 20:26:30

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