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Researchers make new discovery in age-related macular degeneration – Neuroscience News

Summary: Treatment with Humanin G lowered protein levels of inflammatory markers that become elevated in age-related macular degeneration.

source: Archaeological Journals

Inflammatory processes lead to the development of age-related macular degeneration (AMD) – a leading cause of vision loss in the United States.

In this new aging In the study, researchers from the University of California, Irvine and the University of Southern California compared the protein levels of inflammatory markers in normal and AMD retinal pigment hybrid cells (RPE) and explored the effects of Humanin G cell exogenous treatment.

Humanin G (HNG) is a mitochondrial-derived peptide that is cell-protective in AMD and can protect against cellular and mitochondrial stress caused by AMD mitochondrial damage.

“The aim of this study was to test our hypothesis that inflammation-related protein levels are increased in AMD and that treatment with HNG leads to decreased protein levels.”

Humanin G protein levels in plasma of AMD patients and normal subjects were measured using an ELISA assay. Humanin G was added to AMD and normal (control) cybrids derived from clinically characterized AMD patients and normal (control) subjects.

Cell lysates were extracted from untreated AMD, treated with HNG and normal cybrids, and a Luminex XMAP multiplexed assay was used to measure the levels of inflammatory proteins.

The researchers found that there were varying levels of inflammatory proteins between the normal plasma samples and the AMD samples. Compared with control plasma samples, AMD plasma showed higher protein levels of inflammatory markers.

Inflammatory processes lead to the development of age-related macular degeneration (AMD) – a leading cause of vision loss in the United States. The image is in the public domain

However, plasma levels of endogenous Humanin protein were 36.58% lower in AMD patients compared with those in age-matched normal subjects. After treatment with Humanin G, the researchers observed a significant decrease in protein levels of inflammatory markers that were elevated in AMD RPE-transfected hybrid cells.

In conclusion, we present new findings that: a) show reduced human protein levels in AMD versus normal plasma; b) suggest a role for inflammatory markers in the pathogenesis of AMD, and c) highlight the positive effects of Humanin G in reducing inflammation in AMD.”

To the team’s knowledge, this is the first study to report that Humanin protein levels are significantly reduced in AMD patients, thus supporting the pivotal role of Humanin in maintaining tissue homeostasis and normal functioning in the eye.

The researchers concluded, “Our discovery is novel and may contribute to the development of therapeutic tools to reduce inflammation to alleviate the pathology of AMD.”

About this research news related to age-related macular degeneration

author: press office
source: Archaeological Journals
Contact: Press Office – Archaeological Journals
picture: The image is in the public domain

original search: open access.
“Effect of Humanin G (HNG) on inflammation in age-related macular degeneration (AMD)” by Sonali Nashine et al. aging

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Summary

Effect of Humanin G (HNG) on inflammation in age-related macular degeneration (AMD)

Inflammation plays an important role in the aetiology of AMD (age-related macular degeneration). Humanin G (HNG) is a mitochondrial-derived peptide (MDP) that is cell-protective in AMD and can protect against cellular and mitochondrial stress caused by AMD mitochondrial damage.

The aim of this study was to test our hypothesis that inflammation-related protein levels are increased in AMD and that treatment with HNG leads to decreased protein levels. Human protein levels in plasma of AMD patients and normal subjects were measured using an ELISA assay. Humanin G was added to AMD and normal cybrids (control) which had identical nuclei from mitochondrial-deficient ARPE-19 cells but differed in mitochondrial DNA (mtDNA) content derived from clinically characterized AMD patients and normal subjects (control).

Cell lysates were extracted from untreated AMD, treated with HNG and normal cybrids, and a Luminex XMAP multiplexed assay was used to measure the levels of inflammatory proteins. AMD plasma showed lower levels of human protein, but higher protein levels of inflammatory markers compared to control plasma samples.

In AMD RPE cybrid cells, Humanin G CD62E/E-Selectin, CD62P/P-Selectin, ICAM-1, TNF-α, MIP-1α, IFN-γ, IL-1β, IL-13 and IL-17A protein levels decreased, Suggesting that Humanin G may rescue from mtDNA-mediated inflammation in AMD cybrids.

In conclusion, we present new results which are: a) showing reduced human protein levels in AMD plasma versus normal plasma; B) indicate the role of inflammatory markers in the pathogenesis of AMD, and C) highlight the positive effects of Humanin G in reducing inflammation in AMD.

2022-06-03 22:28:40

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